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principles of the collecting and volume of a material of research

Research material were clinical and morphological data of 256 patients who were on stationary inspection and treatment in gynecology departments GKB № 55 and RONTS of N.N.Blohina of the Russian Academy of Medical Science and their blood sera.

At entering in a hospital all patients have been surveyed under the standard plan, including:

1. The collecting of the anamnesis taking into account nameplate data, nationalities, a constant residence, professional and household harmfulnesses, bad habits, features of a food, the family anamnesis, the genesial anamnesis, the transferred diseases. At studying anemnesticheskih data considered the detailed analysis transferred and accompanying extragenital and

Gynecologic diseases. To the careful analysis will subject character menstrual tsiklas the account of such indicators, as age menarhe, to feature of formation of menstrual function,

Duration and obema hemorrhages. Genesial function was estimated on age the beginning of a sexual life, to quantity beremennostej, to their current and an outcome.

2. Physical research of organs of a small basin and a mammary gland.

3. Laboratory researches (the general and biochemical analysis of blood, the general analysis of urine, definition of level СА125).

4. Tool researches: Ouse-tomography of organs of a small basin and an abdominal cavity, a mammography, roentgenography of organs of a thorax.

5. Endoscopic research of organs of a gastroenteric tract,

Including an irrigoscopy and esophagogastroscopy it was spent at all patients with neoplasms of ovaries for an exception of a pathology of a gastroenteric tract (at impossibility applied

Radiological methods of research of a stomach and duodenum).

6. Further inspection of patients extended in the limits necessary for statement of the diagnosis (a computer tomography and magnitnorezonansnaja a tomography vypolsnjalas at patients with widespread tumoral process for innidiation verification.

Special laboratory biochemical methods of research were spent under following schemes.

At all surveyed patients the capture of venous blood on an empty stomach prior to the beginning of treatment any kind was made. Then made unit of Serum from formulated elements of blood. Samples inkubirovalis at a room temperature (18-20) within 30-40 minutes before full formation of a clot. After a retraction of a clot of assay tsentrifugirovali within 10-15 minutes, then Serum merged in secondary test tubes and marked. The received assays froze and stored at temperature - 70 With before reception of the histological conclusion.

As it was already mentioned in chapter 1, the samples used for working out of an adequate method proteomnoj of diagnostics of a cancer, should correspond to strict demands [White et al, 2005]:

1. Absence of statistically significant differences between compared groups, except a sign presence or absence of illness.

2. Control sample should include not only healthy faces, but also the persons, suffering good-quality tumours and not oncologic diseases.

3. Absence of differences in the middle age, differently the found differences can be a consequence not illnesses, and age changes.

4. Absence of differences in techniques of reception and storage of samples of blood sera between compared groups.

Being conformed to the specified demands, in our research 4 groups of patients have been selected:

1. Sick of a cancer of ovaries - the basic group

2. Sick of a good-quality tumour of an ovary - the control.

3. Sick of a hysteromyoma - the control.

4. Healthy women - the control.

Criteria of selection of patients in investigated sample were:

1. Uniformity of an ethnic accessory.

2. Gistologicheski the verified diagnosis.

3. Age - on type "case-control"

After verification of the diagnosis from 256 patients the investigated sample which has included 91 patient taking into account criteria of selection (the table № 1) has been generated.

The table № 1

Investigated sample of patients

Groups

The diagnosis Quantity

Patients

Middle age (minimum - maximum)
I Cancer of ovaries 34 47 (26-70)
II Good-quality tumour of an ovary 14 44 (19-73)
III Hysteromyoma 17 44 (22-56)
IV The healthy 26 41 (21-59)

Apparently in the table № 1, the basic group was made by 34 patients, suffering a cancer of the ovaries, selected in gynecology department RONTS of N.N.Blohina of the Russian Academy of Medical Science.

The second group have made 14 patients suffering

Good-quality neoplasms of the ovaries, selected in

Gynecology department GKB № 55.

The third group was made by 17 patients, suffering a hysteromyoma, selected in gynecology department GKB № 55.

The fourth group was made by 26 patients without a gynecologic pathology ("healthy"), selected at the next planned

Gynecologic inspection in gynecology department GKB № 55.

Middle age of patients in investigated groups was approximately identical - 41-47 years.

2.2.

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A source: NARIMANOVA Metanat RAFIGOVNA. VALUE of DEFINITION of the SERUMAL AMYLOID And 1 In DIFFERENTIAL DIAGNOSTICS of TUMOURS of OVARIES. The dissertation on competition of a scientific degree of the candidate of medical sciences. Moscow - 2014. 2014

More on topic principles of the collecting and volume of a material of research:

  1. 1.1. CONCEPT OF COLLECTING OF MATERIAL EVIDENCES
  2. 2.1. INVESTIGATORY ACTIONS AS APPROPRIATE WAYS COLLECTING OF MATERIAL EVIDENCES
  3. 2.2. OTHER LEGAL PROCEEDINGS AS WAYS OF COLLECTING MATERIAL EVIDENCES
  4. 3.2. WAYS OF COLLECTING OF MATERIAL EVIDENCES IN JUDICIAL CRIMINAL MANUFACTURE
  5. 1.2. THE GENERAL CHARACTERISTIC OF WAYS OF COLLECTING OF THE MATERIAL PROOFS IN CRIMINAL TRIAL
  6. Volume of the investigated material
  7. the theory and practice of collecting of indemnification of non-material harm to legal bodies and individual businessmen.
  8. Chapter 1 CONCEPT AND WAYS OF COLLECTING OF MATERIAL EVIDENCES OF CRIMINAL TRIAL RUSSIA
  9. 3.1. FEATURES OF WAYS OF COLLECTING OF THE MATERIAL PROOFS IN THE STAGE OF EXCITATION OF CRIMINAL CASE
  10. Chapter 2 INVESTIGATORY AND OTHER LEGAL PROCEEDINGS AS WAYS OF COLLECTING OF MATERIAL EVIDENCES IN THE CRIMINAL TRIAL
  11. Chapter 3 WAYS OF COLLECTING OF MATERIAL EVIDENCES IN SEPARATE HUNDRED DIJAH CRIMINAL TRIAL
  12. volume, the organisation and research techniques
  13. volume, the organisation and research techniques
  14. MAMEDOV RAMIL JAGUBOVICH. WAYS OF COLLECTING OF MATERIAL EVIDENCES IN THE RUSSIAN CRIMINAL PROCESS. The dissertation on competition of a scientific degree of the candidate Jurisprudence. Krasnodar - 2016, 2016
  15. objects and volume of clinical research
  16. volume and structure of research work
  17. Chapter 4: Research of molekuljarno-genetic markers in not changed mucous stumps of a stomach at the patients operated concerning a cancer in volume of a resection.
  18. methods and a research material
  19. a research Material
  20. selection of a material of research