2.2.3.2. Allocation total RNK from grudok bumblebees and reverse transcription
ekstraktsiju total RNK from thoraxes Bombus terrestris carried out "trizolnym" a method. Qualities of a preparation received RNK checked with the help elketroforeza (fig. 22).
Fig. 22. elektroforegramma total RNK from thoraxes B. terrestris.
On given elektroforegramme it is visible, that quantity 28S rrnk above, than 18S rrnk it means that there was no degradation RNK under the influence of RNKaz. Also there is no DNA admixing. Thus, given sample RNK approaches for the further operations (reverse transcription and then PCR-reaction).
For synthesis kdnk the anatropic transcriptase of virus Molony lejkoza mice M-MuLv was used. Enzyme shows 5 ’ ^3 ’ polimeraznuju activity, effective only concerning templates RNK. In quality zatravochnogo prajmera it was used oligo - (bt) 2о prajmer. Use
prajmerov the given phylum allows to synthesise moleuly kdnk exclusively from mrnk. During reverse transcription the wide panel of products of DNA of various length (fig. 23 is formed.). Received kdnk used for PCR-reactions.
Fig. 23. elektroforegramma kdnk from thoraxes B. terrestris.
More on topic 2.2.3.2. Allocation total RNK from grudok bumblebees and reverse transcription:
- allocation total RNK
- reverse transcription Carrying out
- allocation mitohondry from flying muscles of bumblebees
- Intensity of consumption of oxygen and allocation of carbonic gas by bumblebees
- the Gene the Runt-bound factor of a transcription 3 (RUNX3).
- the Appendix of 1 Rule of a regular practical transcription (transliteration) OP from the French language on Russian 1
- 2.2.2. Influence of fungicides on biopower processes in mitohondrijah flying muscles of bumblebees
- 2.2.1.2. The characteristic catabolic activity mitohondry flying muscles of bumblebees
- calcium transport through an inner membrane mitohondry flying muscles of bumblebees
- 2.2. З.1. Working out prajmerov for genes of bumblebees
- the Oxidative metabolism mitohondry flying muscles of bumblebees
- SECTION III. THE TOTAL TEST AT THE RATE «ECONOMY HISTORY»
- Production of awake forms of oxygen mitohondrijami flying muscles of bumblebees
- 7. A principle of total quality management.
- Influence of long flight on an expression of genes key antioksidantnyh enzymes of bumblebees
- 7. Share GPT in the general total environmental contamination.
- the sample Total amount
- results of total diagnostics of abilities of self-organising of students of pedagogical directions
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