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cultivation of cultures of zooblasts


Cultures of human cancer cells HeLa (Paneko, Russia) and cages of nephroses of embryos of a pig (SPEV), kindly given to S.A.Staroverovym, grew up on the medium for culture of fabrics RPMI-1640 from 10 % embrionalnoj whey of calfs and an antibiotic ampicillin (100
About
Mkg/ml) in a couveuse at 37 With and 5 % maintenance СО2.
After processing of 1 % tripsinom, cages were three times washed and resuspendirovalis in Hanks's solution (the Bioprize, Russia) and were lead up to a final concentration 2х105 kl/ml. Inventory of quantity of cages SPEV and HeLa spent in chamber Gorjaeva.
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A source: GUSEV Jury Sergeevich. FRAME And FUNCTIONS of PROTEIN VirE2 In TRANSMISSION otsdnk VEUKARIOTICHESSKY CAGES. 2014

More on topic cultivation of cultures of zooblasts:

  1. 2.1. kultivirovanie the cultures forbidden to cultivation
  2. 3.11.1 Experiments on transmission otsdnk with participation of protein VirE2 and its complexes through membranes of zooblasts
  3. Measurement of fluorescence at zooblasts
  4. 4.1. Interaction EK - GMK in the conditions of joint cultivation
  5. technologies of reproduction and cultivation klarievogo a catfish
  6. Cultivation by a method of directional crystallisation
  7. 1.6. Cultivation of monocrystals of germanium and paratellurita from a melt
  8. a choice of optimum density of alighting at cultivation commodity klarievogo a catfish
  9. African klarisvyj a catfish - perspective object of commodity cultivation in UZV
  10. 2.1.14. Definition of sensitivity of cultures of mushrooms to antifungalnym to preparations.
  11. CHAPTER 4. ECONOMIC EFFICIENCY OF CULTIVATION KLARIEVOGO OF THE CATFISH IN UZV AT ASTATICHNOM THE THERMOMODE
  12. Cultivation by a method Chohralsky
  13. a choice optimum astatichnogo termorezhnma at cultivation klarievogo a catfish.
  14. the maintenance multinuclear EK in cultures endotelija from regions NPA and VPA
  15. cultivation of crystals paratellurita an expedient Chohralsky
  16. 2.2. Cultures eukarioticheskih crates
  17. TEORETIKO-FILOSOFSKIEASPEKTY SOTSIODINAMIKI CULTURES