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2.13. The MTT-TEST

Viability of cellular culture after action estimated by means of the MTT-TEST [28]. For this purpose in rostovuju medium imported 3 (4,5 dimetil-2-tiazolil)-2,5-difenil-2N-tetrazolija bromide (the MTT-REAGENT, Alfa Aesar, the Great Britain) to terminating concentration of 0,5 mg/ml and inkubirovali crates during 4 ch.

Further incubatory medium selected, and crystals formed coloured MtT-formazana dissolved in 200 mkl DMSO. Measuring of an optical density of contents by everyone lunki was spent on tablet spectrophotometer Synergy MX on a wave length by of 570 nanometers. Viability of crates estimated under the relation of value of an optical density of a solution formazana in each test to the control (without an irradiation). The gained dependences "dose-effect" used for calculation of the dose leading to inhibition of growth of culture of crates on 50 % (ИК50).
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A source: Shiljagina Natalia Jurevna. EXAMINATION TETRAARILTETRATSIANOPORFIRAZINOV As POTENTIAL PHOTOSENSITIZERS FOR PHOTODYNAMIC THERAPY And FLUORESCENT DIAGNOSTICS. 2014

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