2.13. The MTT-TEST
Viability of cellular culture after action estimated by means of the MTT-TEST [28]. For this purpose in rostovuju medium imported 3 (4,5 dimetil-2-tiazolil)-2,5-difenil-2N-tetrazolija bromide (the MTT-REAGENT, Alfa Aesar, the Great Britain) to terminating concentration of 0,5 mg/ml and inkubirovali crates during 4 ch.
Further incubatory medium selected, and crystals formed coloured MtT-formazana dissolved in 200 mkl DMSO. Measuring of an optical density of contents by everyone lunki was spent on tablet spectrophotometer Synergy MX on a wave length by of 570 nanometers. Viability of crates estimated under the relation of value of an optical density of a solution formazana in each test to the control (without an irradiation). The gained dependences "dose-effect" used for calculation of the dose leading to inhibition of growth of culture of crates on 50 % (ИК50).More on topic 2.13. The MTT-TEST:
- the Comparative analysis of patients OG with a positive Mantoux test on 2 THOSE and 5 THOSE with patients of a subgroup of the basic group with otritsayotelnoj a Mantoux test on 2 THOSE and positive RM on 5 THOSE
- the BASIC STAGES of the KONSTRUIROVANIJAPEDAGOGICHESKY TEST
- Prognostic value roll-over the test and the GARDEN
- TOOL programmnyesistemy FOR RAZRABOTKII USES of TEST TASKS
- SECTION III. THE TOTAL TEST AT THE RATE «ECONOMY HISTORY»
- the pedagogical test AS the OBJECTIVE WAY OTSENIVANIJA
- Function of definition of an order of generation of test signals
- Ruwe registratie Test 1
- Registratieverfijning Test 1
- the Appendix 1 TEST VERBAL KREATIVNOSTI (RAT) With. MEDNIKA (A.N.Voronina's adaptation, an adult variant)
-
Anthropology -
Biochemistry -
Biocomputer science -
Biological resources -
Biological sciences -
Biophysics -
Biotechnology -
Botany -
Development Biology, embriology -
Ecology -
Embryology and Histology -
Entomology -
Genetics -
Histology, cytology, cellular biology -
Hydrobiology -
Ichthyology -
Micology -
Microbiology -
Molecular biology -
Molecular genetics -
Physiology -
Physiology and biochemistry of plants -
Prohistology -
Radio biology -
Soil science -
Virology -
Zoology -
-
Agricultural sciences -
Architecture and construction -
Art criticism -
Biological sciences -
Chemical sciences -
Cultural science -
Economic sciences -
Engineering science -
Geographical sciences -
Geologo-mineralogical sciences -
Historical sciences -
Jurisprudence -
Management, marketing -
Medical sciences -
Military science -
Pedagogical sciences -
Pharmaceutical sciences -
Philological sciences -
Philosophical sciences -
Physics and mathematics -
Political sciences -
Psychological sciences -
Sciences about the Earth -
Sociological sciences -
Veterinary sciences -