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2.2. Cultures eukarioticheskih crates

tetraariltetratsianoporfirazinov spent Examination of dynamics of accumulation, intracellular localisation, photodynamic activity and specificity of accumulation on cultures tumoral crates of the person and the animals presented in table 2.
Table 2.

The cellular lines used in operation

The cellular

Line

The number under catalogue ATCC The description
А-431 CRL-1555 ™ epidermoidnaja kartsinoma skin of the person
HeLa CCL-2 ™ kartsinoma necks of a uterus of the person
MCF-7 HTB-22 ™ adenokartsinoma a mammary gland of the person
Т24 HTB-4 ™ kartsinoma a bladder of the person
Colo-26 CRL-2638 ™ kartsinoma a thick gut of the mouse


Crates of lines А-431, HeLa and Colo-26 cultivated in the environment of DMEM (PanEko, Russia), crates of lines Т24 and MCF-7 — in medium Needle MEM (PanEko, Russia). Rostovye mediums contained 10 % embrionalnoj the veal whey (HyClone, the USA) and 2 mM L-glutamin (PanEko, Russia). kultivirovanie spent in С02-инкубаторе at 37С and atmosphere of 5 % С02. At each stage passirovanija crates handled 0,25 % a solution tripsina-EdTa (PanEko, Russia) [47].

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A source: Shiljagina Natalia Jurevna. EXAMINATION TETRAARILTETRATSIANOPORFIRAZINOV As POTENTIAL PHOTOSENSITIZERS FOR PHOTODYNAMIC THERAPY And FLUORESCENT DIAGNOSTICS. 2014

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