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definition of toxicity of materials

Cages inkubirovalis with the listed materials at various concentrations during 10 mines, 1 hour and 3 hours at temperature 36±1°С for definition of influence of investigated materials on membranes and kernels of cages bukkalnogo an epithelium, and also optimum conditions for the basic experiment.

As a check sample was the sample without the material addition, containing is taken at the same temperature.

2.7.3. Definition of change of toxicity of a preparation at addition intertseptorov (fullerena or caffeine)

For the basic stage of experiments in the separate tube admixed 0.05 ml of the received solution of a preparation about 0.95 ml of solution fullerena or caffeine (having received 110-5 mol/l solution doksorubitsina or 2 10-5 mol/l solution bromic etidija or a proflavine about 1.32 10-4 mol/l fullerena or 9.510 mol/l of caffeine). In 0.2 ml of a preparation added 3.8 ml of buffer solution, having received solution of a preparation for titration (1 10-5 mol/l for solution doksorubitsina and 2 10-5 mol/l for solution bromic etidija or a proflavine).

In 0.5 ml of cellular suspension added 0.5 ml of an admixture preparatintertseptor with various concentration fullerena or caffeine (starting concentration fullerena before titration has compounded 6 mol/l, caffeine -

-3

4.75 10 mol/l). Then cages inkubirovalis in the received solutions within 1 hour at temperature 36±1°С.

As the positive control cages, which inkubirovalis only in preparation solution, as negative - the cages containing without a preparation and intertseptorov at the same temperature were considered.

1.5. A spectophotometery method

Spectra have been removed with use of biradial spectrophotometer SQ-4802 (UNICO, the USA), allowing to carry out measurements in a range of lengths of waves of 280-800 nanometers. Removal of spectra was spent in polimetilakrilatnyh the ditches (Spain) having length of an optical path 1 sm, at a room temperature.

1.6. Methods of statistical data processing

Calculations of average values and average standard errors effected in program Microsoft Office Excel and SigmaPlot. Reliability of distinctions between average values of the received indicators and kontroljami estimated by means of t-criterion Stjudenta. For the purpose of an assessment of statistical communication between the received data and influence of various factors, in program SigmaStat

The procedures presented in the given chapter on endocellular microelectrophoresis, an irradiating on frequency of 3.7 GHz and also have been in detail stated and used by electrical and magnetic components EMI in following works [19, 20, 127].

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A source: Skamrova Galina Borisovna. COMBINED ACTION of WEAK MICROWAVE RADIANCE And DNK-SVJAZYVAJUSHCHIHSJA PREPARATIONS ON CAGES BUKKALNOGO of the EPITHELIUM of the PERSON. 2014

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