<<
>>

2.5. Examination of dynamics of accumulation tetraariltetratsiano - porfirazinov tumoral crates

For an estimate of dynamics of accumulation fluoroforov crates sowed on 96 lunochnye tablets in quantity 3x10 crates on lunku and grew up within night. In lunki imported on 200 mkl mediums with a preparation (concentration 10 microns) and inkubirovali during various intervals of time till 10 o'clock.

For definition dozovoj dependences of accumulation fluoroforov in lunki similarly prepared tablet with crates imported on 200 mkl mediums with a preparation to concentrations from 0 to 350 microns. The exposure made 2 hours.

After the termination inkubatsii crates washed out PBS and flooded standard rostovoj medium. Intensity of fluorescence measured by means of tablet spectrophotometer-spectrofluorometer Synergy Mx at excitation on a wave length of 590 nanometers.

<< | >>
A source: Shiljagina Natalia Jurevna. EXAMINATION TETRAARILTETRATSIANOPORFIRAZINOV As POTENTIAL PHOTOSENSITIZERS FOR PHOTODYNAMIC THERAPY And FLUORESCENT DIAGNOSTICS. 2014

More on topic 2.5. Examination of dynamics of accumulation tetraariltetratsiano - porfirazinov tumoral crates:

  1. 2.8. The analysis of photodynamic activity tetraariltetratsiano - porfirazinov concerning tumoral crates in culture
  2. 3.2. Examination of dynamics of accumulation and intracellular localisation tetraariltetratsianoporfirazinov
  3. 3.3. The analysis of photodynamic activity tetraariltetratsianoporfirazinov concerning tumoral crates in culture
  4. 2.15. Examination of specificity of accumulation in a tumour tetraariltetratsianoporfirazinov in experiment ex vivo
  5. 3.3. Examination of pharmacokinetics and specificity of accumulation in a tumour tetraariltetratsianoporfirazinov in experiments in vivo
  6. 2.14. Examination of pharmacokinetics and specificity of accumulation in a tumour tetraariltetratsianoporfirazinov in experiment in vivo
  7. 2.2. Cultures eukarioticheskih crates
  8. 2.2. Animals and tumoral model
  9. 1.3. Fluorescent molecular curls in an estimate of the answer of crates on photodynamic action
  10. CHAPTER 2. EXPERIMENTAL TECHNIQUES, OBJECTS OF EXAMINATION AND METHODS OF THE ANALYSIS OF EFFECTS OF EXAMINATION
  11. 5.2. Initial accumulation of capital: sources, methods and results
  12. 5.4. Conditions of initial accumulation of capital in Russia